This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Watanabe, K Articles by Hayashi, S Search for Related Content PubMed PubMed Citation Articles by Watanabe, K Articles by Hayashi, S Agricola Articles by Watanabe, K Articles by Hayashi, S

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1987 November; 53(11): 2686-2688

Preparation and regeneration of bacteriophage PL-1 enzyme-induced Lactobacillus casei protoplasts.

Faculty of Pharmaceutical Science, Fukuoka University, Jonan-ku, Japan.

ABSTRACT

Lactobacillus casei ATCC 27092 protoplasts were obtained by treatment with a bacteriolytic enzyme, which was produced in the PL-1 phage lysates of this bacterium, at 37 degrees C for 1.5 h in 50 mM Tris hydrochloride buffer (pH 7.2) containing 20% sucrose and 10 mM MgSO4. The protoplasts lacked the cell wall layer but retained the ability to transport L-[3H]glutamine. The frequency of regeneration was about 1%, whereas about 99% of the cells were osmotically sensitive.