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Appl Environ Microbiol. 1987 March; 53(3): 596-597
Copyright © 1987, American Society for Microbiology. All Rights Reserved.

Influence of Soil and Nonsoil Environments on Nodulation by Rhizobium trifolii

David H. Demezas and Peter J. Bottomley^*

¹ Departments of Microbiology and Soil Science, ² Oregon State University, Corvallis, Oregon 97331-3804

ABSTRACT

Indigenous serotypes 1-01 and 2-02 of Rhizobium trifolii occupied similar percentages (18 to 23%) of root nodules on soil-grown subclover (Trifolium subterraneum L.) and were virtually absent (4.5%) from nodules of soil-grown white clover (Trifolium repens L.). In contrast (with the exception of one dilution [10^–4]), serotype 1-01 occupied a substantial portion of nodules (16 to 40%) on white clover seedlings grown on mineral salts agar and exposed to samples of the same soil in the form of a 10-fold dilution series (10^–1 to 10^–5). Under the latter conditions, occupancy of subclover nodules by 1-01 and of nodules of both plant species by 2-02 was consistent with the results obtained with soil-grown plants.

^* Corresponding author.

Present address: Commonwealth Scientific and Industrial Research Organisation, Division of Plant Industry, Canberra, ACT 2601, Australia.

Technical paper no. 7989 of the Agricultural Experiment Station, Oregon State University, Corvallis.