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Appl Environ Microbiol. 1987 March; 53(3): 598-602
Copyright © 1987, American Society for Microbiology. All Rights Reserved.

Measurement of Surface Charge of Baculovirus Polyhedra

Natural Environment Research Council, Institute of Virology, Oxford OX1 3SR, England

ABSTRACT

The isoelectric points of three baculoviruses, Trichoplusia ni nuclear polyhedrosis virus (NPV), T. ni granulosis virus, and Spodoptera littoralis NPV were identified by cell electrophoresis. At neutral pH polyhedra were negatively charged. T. ni NPV polyhedra were reacted with a number of reagents which could potentially attach to or degrade their surface structure. This gave information on the components that contribute to the charge profile of T. ni NPV. This is discussed in relation to the use of polyhedra as biological control agents against insect pests.

^* Corresponding author.