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Appl Environ Microbiol. 1987 May; 53(5): 923-927
Copyright © 1987, American Society for Microbiology. All Rights Reserved.

Plasmid-Determined Systems for Restriction and Modification Activity and Abortive Infection in Streptococcus cremoris

Laboratoire de Recherche de Technologie Laitière, Institut National de la Recherche Agronomique, 35042 Rennes, France

ABSTRACT

Streptococcus cremoris strain IL964 possessed a restriction and modification (R/M) activity which resulted in a bacteriophage efficiency of plating of 5 x 10^–6. Phage sensitivity of protoplast-induced plasmid-cured derivatives indicated that two plasmids called pIL103 (5.7 kilobases) and pIL107 (15.2 kilobases) were each coding for one R/M system. Plasmid pIL103-encoded R/M was ascertained by transfer into the plasmid-free, R^–/M^– strain IL1403 of S. lactis, using protoplast cotransformation. This procedure failed for pIL107 because of some degree of incompatibility between pIL107 and the indicator plasmid pHV1301 used in cotransformation experiments. We also observed that plasmid pIL105 (8.7 kilobases) which showed no incidence on phage sensitivity in the parental strain IL964, mediated abortive infection in strain IL1403. In 97% of the infected cells, the phage infection was abortive, while in the remaining 3% phages were produced with a decreased burst size (50 instead of 180).

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