This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Vesper, S. J. Search for Related Content PubMed PubMed Citation Articles by Vesper, S. J. Agricola Articles by Vesper, S. J.

Next Article 

Appl Environ Microbiol. 1987 July; 53(7): 1397-1405
Copyright © 1987, American Society for Microbiology. All Rights Reserved.

Production of Pili (Fimbriae) by Pseudomonas fluorescens and Correlation with Attachment to Corn Roots

Battelle-Kettering Laboratory, Yellow Springs, Ohio 45387

ABSTRACT

Pseudomonas fluorescens isolates 13525 and 2-79 were grown in Luria broth and low-nutrient medium (LNM). Pililike fibrils were very rarely produced in Luria broth but were abundantly produced in LNM. In LNM the pili were peritrichously distributed and had diameters ranging from 3 to 8 nm. Pili were purified from strain 2-79, and the pilin subunit was found to have a molecular weight of about 34,000. Strain 2-79 produced two colony types on Luria agar, nonmucoidal and mucoidal. Cells in LNM cultures of the nonmucoidal colony type were highly piliated, and cells from the mucoidal type were nearly devoid of pili. The presence of pili on nonmucoidal isolate 2-79 was quantitatively correlated with hydrophobic attachment to polystyrene, hemagglutination, and attachment to corn roots.

Contribution no. 920 of the Battelle-Kettering Laboratory.

This article has been cited by other articles:

• Barak, J. D., Gorski, L., Naraghi-Arani, P., Charkowski, A. O. (2005). Salmonella enterica Virulence Genes Are Required for Bacterial Attachment to Plant Tissue. Appl. Environ. Microbiol. 71: 5685-5691 [Abstract] [Full Text]  
• Whipps, J. M. (2001). Microbial interactions and biocontrol in the rhizosphere. J Exp Bot 52: 487-511 [Abstract] [Full Text]  
• Roberts, D. P., Dery, P. D., Yucel, I., Buyer, J., Holtman, M. A., Kobayashi, D. Y. (1999). Role of pfkA and General Carbohydrate Catabolism in Seed Colonization by Enterobacter cloacae. Appl. Environ. Microbiol. 65: 2513-2519 [Abstract] [Full Text]  
• DeFlaun, M. F., Oppenheimer, S. R., Streger, S., Condee, C. W., Fletcher, M. (1999). Alterations in Adhesion, Transport, and Membrane Characteristics in an Adhesion-Deficient Pseudomonad. Appl. Environ. Microbiol. 65: 759-765 [Abstract] [Full Text]  
• Matthysse, A. G., McMahan, S. (1998). Root Colonization by Agrobacterium tumefaciens Is Reduced in cel, attB, attD, and attR Mutants. Appl. Environ. Microbiol. 64: 2341-2345 [Abstract] [Full Text]