This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Carbonell, L F Articles by Miller, L K Search for Related Content PubMed PubMed Citation Articles by Carbonell, L F Articles by Miller, L K Agricola Articles by Carbonell, L F Articles by Miller, L K

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1987 July; 53(7): 1412-1417

Baculovirus interaction with nontarget organisms: a virus-borne reporter gene is not expressed in two mammalian cell lines.

Department of Genetics, University of Georgia, Athens 30602.

ABSTRACT

The safety of baculoviruses with respect to mammalian species was studied by using a genetically engineered recombinant of Autographa californica nuclear polyhedrosis virus. This recombinant contains the chloramphenicol acetyltransferase (CAT) gene under the control of a mammalian-active promoter and expresses substantial levels of CAT activity on infection of permissive and nonpermissive insect cells (L.F. Carbonell, M.J. Klowden, and L.K. Miller, J. Virol. 56:153-160, 1985). Extremely low levels of CAT activity were detected in mouse and human cell lines that were continuously exposed to the A. californica nuclear polyhedrosis virus recombinant. The appearance of CAT was not inhibited by cycloheximide. Isopycnic centrifugation of purified inoculum showed that a low level of CAT activity was associated with the insect-derived viral particles. Thus, the observed CAT activity is carried into the cells with the virus inoculum, and active expression of the baculovirus-borne CAT gene is not observed in either cell line. The inability of the CAT gene to be expressed in these cell lines with this model system provides additional assurance of the safety of insect baculoviruses with respect to mammalian species.

This article has been cited by other articles:

• Kenoutis, C., Efrose, R. C., Swevers, L., Lavdas, A. A., Gaitanou, M., Matsas, R., Iatrou, K. (2006). Baculovirus-mediated gene delivery into Mammalian cells does not alter their transcriptional and differentiating potential but is accompanied by early viral gene expression.. J. Virol. 80: 4135-4146 [Abstract] [Full Text]  
• Liang, C., Song, J., Chen, X. (2005). The GP64 protein of Autographa californica multiple nucleopolyhedrovirus rescues Helicoverpa armigera nucleopolyhedrovirus transduction in mammalian cells. J. Gen. Virol. 86: 1629-1635 [Abstract] [Full Text]  
• Dai, X., Willis, L. G., Huijskens, I., Palli, S. R., Theilmann, D. A. (2004). The acidic activation domains of the baculovirus transactivators IE1 and IE0 are functional for transcriptional activation in both insect and mammalian cells. J. Gen. Virol. 85: 573-582 [Abstract] [Full Text]  
• Oomens, A. G. P., Wertz, G. W. (2004). The Baculovirus GP64 Protein Mediates Highly Stable Infectivity of a Human Respiratory Syncytial Virus Lacking Its Homologous Transmembrane Glycoproteins. J. Virol. 78: 124-135 [Abstract] [Full Text]  
• Gronowski, A. M., Hilbert, D. M., Sheehan, K. C. F., Garotta, G., Schreiber, R. D. (1999). Baculovirus Stimulates Antiviral Effects in Mammalian Cells. J. Virol. 73: 9944-9951 [Abstract] [Full Text]  
• Condreay, J. P., Witherspoon, S. M., Clay, W. C., Kost, T. A. (1999). Transient and stable gene expression in mammalian cells transduced with a recombinant baculovirus vector. Proc. Natl. Acad. Sci. USA 96: 127-132 [Abstract] [Full Text]  
• Sarkis, C., Serguera, C., Petres, S., Buchet, D., Ridet, J.-L., Edelman, L., Mallet, J. (2000). Efficient transduction of neural cells in vitro and in vivo by a baculovirus-derived vector. Proc. Natl. Acad. Sci. USA 97: 14638-14643 [Abstract] [Full Text]