This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Cooper, J. E. Articles by Thompson, J. K. Search for Related Content PubMed PubMed Citation Articles by Cooper, J. E. Articles by Thompson, J. K. Agricola Articles by Cooper, J. E. Articles by Thompson, J. K.

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1987 July; 53(7): 1705-1707
Copyright © 1987, American Society for Microbiology. All Rights Reserved.

Identification of Lotus Rhizobia by Direct DNA Hybridization of Crushed Root Nodules

Agricultural and Food Bacteriology Research Division, Department of Agriculture for Northern Ireland, Belfast BT9 5PX, Northern Ireland

ABSTRACT

Hybridization of crushed Lotus pedunculatus root nodules with ³²P-labeled total genomic DNA probes was used to identify Rhizobium loti and Bradyrhizobium sp. (Lotus rhizobia). Probes always hybridized with homologous target DNA and frequently with DNAs of other strains from the same genus. Intergeneric hybridization did not occur. Results were comparable to those from colony hybridization.

^* Corresponding author.