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Appl Environ Microbiol. 1987 September; 53(9): 2256-2259

Detection of hemolytic Listeria monocytogenes by using DNA colony hybridization.

Division of Microbiology, Food and Drug Administration, Washington, D.C. 20204.

ABSTRACT

A fragment of about 500 base pairs of the beta-hemolysin gene from Listeria monocytogenes was used to screen different bacterial strains by DNA colony hybridization. The cells in the colonies were lysed by microwaves in the presence of sodium hydroxide. Of 52 different strains of Listeria species screened, only the DNA from beta-hemolytic (CAMP-positive) strains of L. monocytogenes hybridized with this probe.

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