AEM
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Cosby, W M
Right arrow Articles by Dobrogosz, W J
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cosby, W M
Right arrow Articles by Dobrogosz, W J
Agricola
Right arrow Articles by Cosby, W M
Right arrow Articles by Dobrogosz, W J

Next Article 

Appl Environ Microbiol. 1988 November; 54(11): 2599-2602

Formation, regeneration, and transfection of Lactobacillus plantarum protoplasts.

W M Cosby, I A Casas and W J Dobrogosz

Department of Microbiology, North Carolina State University, Raleigh 27695-7615.

ABSTRACT

Protoplasts of L. plantarum were produced by mutanolysin-lysozyme digestion at 50 degrees C and regenerated at a frequency of 1.6 to 3.8%. The addition of Tween 80 to the growth medium increased the length of time required for protoplast formation. When transfected with bacteriophage B2 DNA, transfection efficiencies ranged from 25 to 230 PFU/microgram of DNA and from 2.2 X 10(-5) to 4.7 X 10(-4) PFU per recovered protoplast. Total transfectant yield was 3.7 X 10(2) to 3.4 X 10(3) per treatment. Transformations with plasmid DNA were unsuccessful.

Appl Environ Microbiol. 1988 November; 54(11): 2599-2602






Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
J. Bacteriol. Microbiol. Mol. Biol. Rev. Eukaryot. Cell All ASM Journals

Copyright © 1988 by the American Society for Microbiology. All rights reserved.