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Appl Environ Microbiol. 1988 April; 54(4): 1039-1045
Copyright © 1988, American Society for Microbiology. All Rights Reserved.

Circular Permutation of the Genome of a Temperate Bacteriophage from Streptococcus cremoris BK5

¹ Russell Grimwade School of Biochemistry, University of Melbourne, Parkville, Victoria 3052, and Division of Food Processing, Dairy Research Laboratory, Commonwealth Scientific and Industrial Research Organization, P.O. Box 20, Highett, Victoria 3190, ² Australia

ABSTRACT

The temperate bacteriophage BK5-T was isolated from Streptococcus cremoris BK5 by induction with mitomycin C. Electron microscopy revealed that BK5-T DNA consists of linear molecules, ranging in size from 39.7 to 46 kilobase pairs. Restriction analysis of self-ligated BK5-T DNA showed that the ends of the DNA were not cohesive. The EcoRI restriction fragments of the phage genome were cloned into pACYC184. Restriction enzyme analysis of both the phage DNA and the cloned EcoRI fragments with EcoRI, BstEII, PstI, ClaI, and XbaI yielded a 37.6-kilobase-pair-long circular restriction map for the phage genome. It was concluded that the BK5-T DNA molecules in the population differ in their sequence by a circular permutation and that individual DNA molecules are terminally redundant. The map location of the sites at which packaging of BK5-T DNA into phage heads is initiated (pac) and at which the phage integrates into the bacterial chromosome (att) were established.

^* Corresponding author.

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