This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Lundin, R E Articles by Sacks, L E Search for Related Content PubMed PubMed Citation Articles by Lundin, R E Articles by Sacks, L E Agricola Articles by Lundin, R E Articles by Sacks, L E

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1988 April; 54(4): 923-928

High-resolution solid-state 13C nuclear magnetic resonance of bacterial spores: identification of the alpha-carbon signal of dipicolinic acid.

Western Regional Research Center, U.S. Department of Agriculture, Berkeley, California 94710.

ABSTRACT

Natural-abundance solid-state 13C nuclear magnetic resonance spectra were obtained for bacterial spores for the first time by using the technique of cross-polarization magic-angle-spinning nuclear magnetic resonance spectroscopy. A resonance at about 150 ppm, detectable in spore samples having a Mn content of less than 0.05%, was consistent with an identification as the alpha-carbon signal of calcium dipicolinate; this signal was missing from a spore sample treated with acid to release dipicolinate and from a spore coat preparation. Carbohydrate peaks were particularly intense in spores and coat preparations of Bacillus macerans. Signals ascribable to beta-hydroxybutyrate were prominent in a B. cereus sample.

This article has been cited by other articles:

• Leuschner, R. G. K., Lillford, P. J. (2000). Effects of hydration on molecular mobility in phase-bright Bacillus subtilis spores. Microbiology 146: 49-55 [Abstract] [Full Text]