AEM
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kamekura, M.
Right arrow Articles by Kushner, D. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kamekura, M.
Right arrow Articles by Kushner, D. J.
Agricola
Right arrow Articles by Kamekura, M.
Right arrow Articles by Kushner, D. J.

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1988 April; 54(4): 990-995
Copyright © 1988, American Society for Microbiology. All Rights Reserved.

Lysis of Halobacteria in Bacto-Peptone by Bile Acids

Masahiro Kamekura*, Dieter Oesterhelt, Rebecca Wallace, Peter Anderson and Donn J. Kushner

1 Noda Institute for Scientific Research, Noda, Chiba 278, Japan; Max Planck Institut für Biochemie, 8033 Martinsried bei München, Federal Republic of Germany2; and Department of Biology3 and Department of Biochemistry, 4 University of Ottawa, Ottawa, Ontario, Canada K1N 6N5

ABSTRACT

All tested strains of halophilic archaebacteria of the genera Halobacterium, Haloarcula, Haloferax, and Natronobacterium lysed in 1% Bacto-Peptone (Difco) containing 25% NaCl, whereas no lysis was observed with other strains belonging to archaebacteria of the genera Halococcus, Natronococcus, and Sulfolobus, methanogenic bacteria, and moderately halophilic eubacteria. Substances in Bacto-Peptone which caused lysis of halobacteria were purified and identified as taurocholic acid and glycocholic acid. High-performance liquid chromatography analyses of peptones revealed that Bacto-Peptone contained nine different bile acids, with a total content of 9.53 mg/g, whereas much lower amounts were found in Peptone Bacteriological Technical (Difco) and Oxoid Peptone. Different kinds of peptones can be used to distinguish halophilic eubacteria and archaebacteria in mixed cultures from hypersaline environments.

FOOTNOTES

* Corresponding author.

Appl Environ Microbiol. 1988 April; 54(4): 990-995
Copyright © 1988, American Society for Microbiology. All Rights Reserved.





Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
J. Bacteriol. Microbiol. Mol. Biol. Rev. Eukaryot. Cell All ASM Journals

Copyright © 1988 by the American Society for Microbiology. All rights reserved.