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Appl Environ Microbiol. 1988 May; 54(5): 1302-1303

Application of an enzyme-linked immunosorbent assay for screening of T-2 toxin-producing Fusarium spp.

S Nagayama, O Kawamura, K Ohtani, J C Ryu, D Latus, L Sudheim and Y Ueno

Department of Toxicology and Microbial Chemistry, Science University of Tokyo, Japan.

ABSTRACT

Culture filtrates of Fusarium species were subjected without clean-up procedures to an indirect competitive enzyme-linked immunosorbent assay with anti-T-2 toxin monoclonal antibody. Fusarium sporotrichioides, F. poae, F. tricinctum, and F. culmorum strains were positive for T-2 toxin, with a minimum detection limit of 5 pg per assay (100 pg/ml of culture filtrate), and the assay data correlated well with the gas-liquid chromatographic data.

Appl Environ Microbiol. 1988 May; 54(5): 1302-1303






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