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Appl Environ Microbiol. 1988 July; 54(7): 1766-1769
Copyright © 1988, American Society for Microbiology. All Rights Reserved.

Nitrile Hydratase-Catalyzed Production of Nicotinamide from 3-Cyanopyridine in Rhodococcus rhodochrous J1

Toru Nagasawa*, Caluwadewa Deepal Mathew{dagger}, Jacques Mauger and Hideaki Yamada

Department of Agricultural Chemistry, Kyoto University, Kitashirakawa, Oiwake-cho, Sakyo-ku, Kyoto 606, Japan

ABSTRACT

Nitrile hydratase, which occurs abundantly in cells of Rhodococcus rhodochrous J1 isolated from soil samples, catalyzes the hydration of 3-cyanopyridine to nicotinamide. By using resting cells, the reaction conditions for nicotinamide production were optimized. Under the optimum conditions, 100% of the added 12 M 3-cyanopyridine was converted to nicotinamide without the formation of nicotinic acid, and the highest yield achieved was 1,465 g of nicotinamide per liter of reaction mixture containing resting cells (1.48 g as dry cell weight) in 9 h. The nicotinamide produced was crystallized and then identified physicochemically. The further conversion of the nicotinamide to nicotinic acid was due to the low activity of nicotinamide as a substrate for the amidase(s) present in this organism.

FOOTNOTES

* Corresponding author.

{dagger} Present address: Department of Biochemistry, Faculty of Medicine, University of Colombo, Colombo 8, Sri Lanka.

Appl Environ Microbiol. 1988 July; 54(7): 1766-1769
Copyright © 1988, American Society for Microbiology. All Rights Reserved.



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Copyright © 1988 by the American Society for Microbiology. All rights reserved.