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Appl Environ Microbiol. 1988 August; 54(8): 1901-1906

Metabolic processes involved in repair of Escherichia coli cells damaged by exposure to acid mine water.

Division of Plant and Soil Sciences, West Virginia University, Morgantown 26506-6057.

ABSTRACT

Escherichia coli was stressed by exposure to filter-sterilized acid mine water. Synthetic processes required for repair of sublethally injured survivors were studied by the addition of specific metabolic inhibitors to a resuscitation broth. Inhibitors of protein, RNA, DNA, lipid, and peptidoglycan synthesis as well as uncouplers and inhibitors of electron transport and ATPase activity were used. Acid mine water injury was severe, causing damage to the outer and cytoplasmic membranes. Repair of sublethally injured cells required protein, RNA, and lipid synthesis as well as a proton motive force.