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Appl Environ Microbiol. 1989 January; 55(1): 266-267
Copyright © 1989, American Society for Microbiology. All Rights Reserved.

Cytokinins in Azotobacter vinelandii Culture Medium

Barbara J. Taller* and Tit-Yee Wong

Department of Biology, Memphis State University, Memphis, Tennessee 38152

ABSTRACT

Azotobacter vinelandii OP was grown to stationary phase in defined medium. The cell-free culture medium was analyzed for cytokinin content by XAD-2 and Sephadex LH-20 chromatography, thin-layer chromatography, tobacco callus bioassay, and enzyme immunoassay. Three cytokinin-active fractions were detected and tentatively identified as trans-zeatin, isopentenyladenosine, and isopentenyladenine. The total cytokinin activity was equivalent to 0.75 µg of kinetin per liter.

FOOTNOTES

* Corresponding author.

Appl Environ Microbiol. 1989 January; 55(1): 266-267
Copyright © 1989, American Society for Microbiology. All Rights Reserved.



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Copyright © 1989 by the American Society for Microbiology. All rights reserved.