This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Oldenhuis, R
Right arrow Articles by Witholt, B
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Oldenhuis, R
Right arrow Articles by Witholt, B
Agricola
Right arrow Articles by Oldenhuis, R
Right arrow Articles by Witholt, B

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1989 November; 55(11): 2819-2826

Degradation of chlorinated aliphatic hydrocarbons by Methylosinus trichosporium OB3b expressing soluble methane monooxygenase.

R Oldenhuis, R L Vink, D B Janssen and B Witholt

Department of Biochemistry, University of Groningen, The Netherlands.

ABSTRACT

Degradation of trichloroethylene (TCE) by the methanotrophic bacterium Methylosinus trichosporium OB3b was studied by using cells grown in continuous culture. TCE degradation was a strictly cometabolic process, requiring the presence of a cosubstrate, preferably formate, and oxygen. M. trichosporium OB3b cells degraded TCE only when grown under copper limitation and when the soluble methane monooxygenase was derepressed. During TCE degradation, nearly total dechlorination occurred, as indicated by the production of inorganic chloride, and only traces of 2,2,2-trichloroethanol and trichloroacetaldehyde were produced. TCE degradation proceeded according to first-order kinetics from 0.1 to 0.0002 mM TCE with a rate constant of 2.14 ml min-1 mg of cells-1. TCE concentrations above 0.2 mM inhibited degradation in cell suspensions of 0.42 mg of cells ml-1. Other chlorinated aliphatics were also degraded by M. trichosporium OB3b. Dichloromethane, chloroform, 1,1-dichloroethane, and 1,2-dichloroethane were completely degraded, with the release of stoichiometric amounts of chloride. trans-1,2-Dichloroethylene, cis-1,2-dichloroethylene, and 1,2-dichloropropane were completely converted, but not all the chloride was released because of the formation of chlorinated intermediates, e.g., trans-2,3-dichlorooxirane, cis-2,3-dichlorooxirane, and 2,3-dichloropropanol, respectively. 1,1,1-Trichloroethane, 1,1-dichloroethylene, and 1,3-dichloropropylene were incompletely converted, and the first compound yielded 2,2,2-trichloroethanol as a chlorinated intermediate. The two perchlorinated compounds tested, carbon tetrachloride and tetrachloroethylene, were not converted.

Appl Environ Microbiol. 1989 November; 55(11): 2819-2826




This article has been cited by other articles:

  • Lee, S.-W., Keeney, D. R., Lim, D.-H., Dispirito, A. A., Semrau, J. D. (2006). Mixed Pollutant Degradation by Methylosinus trichosporium OB3b Expressing either Soluble or Particulate Methane Monooxygenase: Can the Tortoise Beat the Hare?. Appl. Environ. Microbiol. 72: 7503-7509 [Abstract] [Full Text]  
  • Erwin, D. P., Erickson, I. K., Delwiche, M. E., Colwell, F. S., Strap, J. L., Crawford, R. L. (2005). Diversity of Oxygenase Genes from Methane- and Ammonia-Oxidizing Bacteria in the Eastern Snake River Plain Aquifer. Appl. Environ. Microbiol. 71: 2016-2025 [Abstract] [Full Text]  
  • Scheutz, C., Mosbaek, H., Kjeldsen, P. (2004). Attenuation of Methane and Volatile Organic Compounds in Landfill Soil Covers. J. Environ. Qual. 33: 61-71 [Abstract] [Full Text]  
  • Sun, B., Griffin, B. M., Ayala-del-Rio, H. L., Hashsham, S. A., Tiedje, J. M. (2002). Microbial Dehalorespiration with 1,1,1-Trichloroethane. Science 298: 1023-1025 [Abstract] [Full Text]  
  • Yagi, O., Hashimoto, A., Iwasaki, K., Nakajima, M. (1999). Aerobic Degradation of 1,1,1-Trichloroethane by Mycobacterium spp. Isolated from Soil. Appl. Environ. Microbiol. 65: 4693-4696 [Abstract] [Full Text]  
  • Miguez, C. B., Shen, C. F., Bourque, D., Guiot, S. R., Groleau, D. (1999). Monitoring Methanotrophic Bacteria in Hybrid Anaerobic-Aerobic Reactors with PCR and a Catabolic Gene Probe. Appl. Environ. Microbiol. 65: 381-388 [Abstract] [Full Text]  
  • Yeager, C. M., Bottomley, P. J., Arp, D. J., Hyman, M. R. (1999). Inactivation of Toluene 2-Monooxygenase in Burkholderia cepacia G4 by Alkynes. Appl. Environ. Microbiol. 65: 632-639 [Abstract] [Full Text]  
  • Shingleton, J. T., Applegate, B. M., Nagel, A. C., Bienkowski, P. R., Sayler, G. S. (1998). Induction of the tod Operon by Trichloroethylene in Pseudomonas putida TVA8. Appl. Environ. Microbiol. 64: 5049-5052 [Abstract] [Full Text]  
  • Ayoubi, P. J., Harker, A. R. (1998). Whole-Cell Kinetics of Trichloroethylene Degradation by Phenol Hydroxylase in a Ralstonia eutropha JMP134 Derivative. Appl. Environ. Microbiol. 64: 4353-4356 [Abstract] [Full Text]  
  • Chu, K.-H., Alvarez-Cohen, L. (1998). Effect of Nitrogen Source on Growth and Trichloroethylene Degradation by Methane-Oxidizing Bacteria. Appl. Environ. Microbiol. 64: 3451-3457 [Abstract] [Full Text]  
  • Lontoh, S., Semrau, J. D. (1998). Methane and Trichloroethylene Degradation by Methylosinus trichosporium OB3b Expressing Particulate Methane Monooxygenase. Appl. Environ. Microbiol. 64: 1106-1114 [Abstract] [Full Text]  
  • Téllez, C. M., Gaus, K. P., Graham, D. W., Arnold, R. G., Guzman, R. Z. (1998). Isolation of Copper Biochelates from Methylosinus trichosporium OB3b and Soluble Methane Monooxygenase Mutants. Appl. Environ. Microbiol. 64: 1115-1122 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»