AEM
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Nelis, H. J.
Right arrow Articles by De Leenheer, A. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nelis, H. J.
Right arrow Articles by De Leenheer, A. P.
Agricola
Right arrow Articles by Nelis, H. J.
Right arrow Articles by De Leenheer, A. P.

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1989 December; 55(12): 3065-3071
Copyright © 1989, American Society for Microbiology. All Rights Reserved.

Profiling and Quantitation of Bacterial Carotenoids by Liquid Chromatography and Photodiode Array Detection

H. J. Nelis and A. P. De Leenheer*

Laboratoria voor Medische Biochemie en voor Klinische Analyse, Harelbekestraat 72, B-9000 Ghent, Belgium

ABSTRACT

An analytical method for the profiling and quantitative determination of carotenoids in bacteria is described. Exhaustive extraction of the pigments from four selected bacterial strains required treatment of the cells with potassium hydroxide or liquefied phenol or both before the addition of the extracting solvent (methanol or diethyl ether). The carotenoids in the extracts were separated by nonaqueous reversed-phase liquid chromatography in conjunction with photodiode array absorption detection. The identity of a peak was considered definitive only when both its retention time and absorption spectrum, before and after chemical reactions, matched those of a reference component. In the absence of the latter, most peaks could be tentatively identified. Two examples illustrate how in the analysis of pigmented bacteria errors may result from using nonchromatographic procedures or liquid chromatographic methods lacking sufficient criteria for peak identification. Carotenoids of interest were determined quantitatively when the authentic reference substance was available or, alternatively, were determined semiquantitatively.

FOOTNOTES

* Corresponding author.

Appl Environ Microbiol. 1989 December; 55(12): 3065-3071
Copyright © 1989, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:



Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
J. Bacteriol. Microbiol. Mol. Biol. Rev. Eukaryot. Cell All ASM Journals

Copyright © 1989 by the American Society for Microbiology. All rights reserved.