Previous Article | Next Article 
Appl Environ Microbiol. 1989 December; 55(12): 3155-3161
Biodegradation of trichloroethylene by Methylosinus trichosporium OB3b.
H C Tsien,
G A Brusseau,
R S Hanson and
L P Waclett
Gray Freshwater Biological Institute, University of Minnesota, Navarre 55392.
ABSTRACT
The methanotroph Methylosinus trichosporium OB3b, a type II methanotroph, degraded trichloroethylene at rates exceeding 1.2 mmol/h per g (dry weight) following the appearance of soluble methane monooxygenase in continuous and batch cultures. Cells capable oxidizing trichloroethylene contained components of soluble methane monooxygenase as demonstrated by Western blot (immunoblot) analysis with antibodies prepared against the purified enzyme. Growth of cultures in a medium containing 0.25 microM or less copper sulfate caused derepression of the synthesis of soluble methane monooxygenase. In these cultures, the specific rates of methane and methanol oxidation did not change during growth, while trichloroethylene oxidation increased with the appearance of soluble methane monooxygenase. M. trichosporium OB3b cells that contained soluble methane monooxygenase also degraded vinyl chloride, 1,1-dichloroethylene, cis-1,2-dichloroethylene, and trans-1,2-dichloroethylene.
Appl Environ Microbiol. 1989 December; 55(12): 3155-3161
This article has been cited by other articles:
-
Erwin, D. P., Erickson, I. K., Delwiche, M. E., Colwell, F. S., Strap, J. L., Crawford, R. L.
(2005). Diversity of Oxygenase Genes from Methane- and Ammonia-Oxidizing Bacteria in the Eastern Snake River Plain Aquifer. Appl. Environ. Microbiol.
71: 2016-2025
[Abstract]
[Full Text]
-
Shiemke, A. K., Arp, D. J., Sayavedra-Soto, L. A.
(2004). Inhibition of Membrane-Bound Methane Monooxygenase and Ammonia Monooxygenase by Diphenyliodonium: Implications for Electron Transfer. J. Bacteriol.
186: 928-937
[Abstract]
[Full Text]
-
Scheutz, C., Mosbaek, H., Kjeldsen, P.
(2004). Attenuation of Methane and Volatile Organic Compounds in Landfill Soil Covers. J. Environ. Qual.
33: 61-71
[Abstract]
[Full Text]
-
Scheutz, C., Kjeldsen, P.
(2004). Environmental Factors Influencing Attenuation of Methane and Hydrochlorofluorocarbons in Landfill Cover Soils. J. Environ. Qual.
33: 72-79
[Abstract]
[Full Text]
-
Yeager, C. M., Bottomley, P. J., Arp, D. J.
(2001). Cytotoxicity Associated with Trichloroethylene Oxidation in Burkholderia cepacia G4. Appl. Environ. Microbiol.
67: 2107-2115
[Abstract]
[Full Text]
-
Wise, M. G., McArthur, J V., Shimkets, L. J.
(1999). Methanotroph Diversity in Landfill Soil: Isolation of Novel Type I and Type II Methanotrophs Whose Presence Was Suggested by Culture-Independent 16S Ribosomal DNA Analysis. Appl. Environ. Microbiol.
65: 4887-4897
[Abstract]
[Full Text]
-
Hamamura, N., Storfa, R. T., Semprini, L., Arp, D. J.
(1999). Diversity in Butane Monooxygenases among Butane-Grown Bacteria. Appl. Environ. Microbiol.
65: 4586-4593
[Abstract]
[Full Text]
-
Miguez, C. B., Shen, C. F., Bourque, D., Guiot, S. R., Groleau, D.
(1999). Monitoring Methanotrophic Bacteria in Hybrid Anaerobic-Aerobic Reactors with PCR and a Catabolic Gene Probe. Appl. Environ. Microbiol.
65: 381-388
[Abstract]
[Full Text]
-
Yeager, C. M., Bottomley, P. J., Arp, D. J., Hyman, M. R.
(1999). Inactivation of Toluene 2-Monooxygenase in Burkholderia cepacia G4 by Alkynes. Appl. Environ. Microbiol.
65: 632-639
[Abstract]
[Full Text]
-
Shingleton, J. T., Applegate, B. M., Nagel, A. C., Bienkowski, P. R., Sayler, G. S.
(1998). Induction of the tod Operon by Trichloroethylene in Pseudomonas putida TVA8. Appl. Environ. Microbiol.
64: 5049-5052
[Abstract]
[Full Text]
-
Chu, K.-H., Alvarez-Cohen, L.
(1998). Effect of Nitrogen Source on Growth and Trichloroethylene Degradation by Methane-Oxidizing Bacteria. Appl. Environ. Microbiol.
64: 3451-3457
[Abstract]
[Full Text]
-
Lontoh, S., Semrau, J. D.
(1998). Methane and Trichloroethylene Degradation by Methylosinus trichosporium OB3b Expressing Particulate Methane Monooxygenase. Appl. Environ. Microbiol.
64: 1106-1114
[Abstract]
[Full Text]