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Appl Environ Microbiol. 1989 December; 55(12): 3229-3230
Copyright © 1989, American Society for Microbiology. All Rights Reserved.

Host Restriction and Transduction in Rhizobium meliloti

Myron N. V. Williams, Shoshana Klein and Ethan R. Signer^*

Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139

ABSTRACT

A host restriction difference exists between Rhizobium meliloti Rm41 and SU47 exists as indicated by the reduce plating efficiency of transducing phage M12h1. Restriction can be attenuated by incubating cells at 42°C for 3 h; this procedure overcomes a block to transduction from SU47 to Rm41.

^* Corresponding author.

Present address: Department of Genetics, Hebrew University, Jerusalem.

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