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Appl Environ Microbiol. 1989 December; 55(12): 3237-3239

Lyophilization of rumen fluid for use in culture media.

Department of Animal Science, Ohio Agricultural Research and Development Center, Ohio State University, Wooster 44691.

ABSTRACT

The supernatant from centrifugation at 1,000 x g of strained rumen fluid was lyophilized, and the residue and sublimate fractions were used to replace fresh rumen fluid in a complete roll tube medium for enumeration of total rumen bacteria. Most of the growth-supporting nutrients in fresh rumen fluid were found in the residue fraction. With one exception, no significant differences were found in total bacterial numbers either by roll tube or most-probable-number procedures when lyophilized rumen fluid residue was substituted for fresh rumen fluid. Lyophilized rumen fluid residue was stable for at least 5 months at room temperature. Rumen fluid supernatant from centrifugation at 1,000 x g had a mean density of 1.005 +/- 0.03 g/ml and contained 1.56% +/- 0.30% dry matter. On the basis of these values, 15.68 mg of lyophilized rumen fluid residue is equivalent to 1 ml of rumen fluid supernatant from centrifugation at 1,000 x g.

This article has been cited by other articles:

• Dehority, B. A., Tirabasso, P. A. (2000). Antibiosis between Ruminal Bacteria and Ruminal Fungi. Appl. Environ. Microbiol. 66: 2921-2927 [Abstract] [Full Text]