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Appl Environ Microbiol. 1989 December; 55(12): 3250-3252

Development of enterobacterium-specific oligonucleotide probes based on the surface-exposed regions of outer membrane proteins.

G Spierings, H Hofstra, J Huis in'T Veld, W Hoekstra and J Tommassen

Institute of Molecular Biology and Medical Biotechnology, State University of Utrecht, The Netherlands.

ABSTRACT

Outer membrane proteins of members of the family Enterobacteriaceae consist of conserved membrane-spanning segments and hypervariable, surface-exposed regions. We demonstrate that the hypervariable DNA segments corresponding to the surface-exposed regions of these proteins can be used to develop specific DNA probes for the identification of members of the family Enterobacteriaceae.

Appl Environ Microbiol. 1989 December; 55(12): 3250-3252






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