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Appl Environ Microbiol. 1989 February; 55(2): 275-278
Copyright © 1989, American Society for Microbiology. All Rights Reserved.

Pyrroloquinoline Quinone-Dependent Cytochrome Reduction in Polyvinyl Alcohol-Degrading Pseudomonas sp. Strain VM15C

Masayuki Shimao*, Syuji Onishi, Nobuo Kato and Chikahiro Sakazawa

Department of Environmental Chemistry and Technology, Tottori University, Tottori 680, Japan

ABSTRACT

A polyvinyl alcohol (PVA) oxidase-deficient mutant of Pseudomonas sp. strain VM15C, strain ND1, was shown to possess PVA dehydrogenase, in which pyrroloquinoline quinone (PQQ) functions as a coenzyme. The mutant grew on PVA and required PQQ for utilization of PVA as an essential growth factor. Incubation of the membrane fraction of the mutant with PVA caused cytochrome reduction of the fraction. Furthermore, it was found that in spite of the presence of PVA oxidase, the membrane fraction of strain VM15C grown on glucose without PQQ required PQQ for cytochrome reduction during incubation with PVA. The results provide evidence that PVA dehydrogenase couples with the electron transport chain of PVA-degrading bacteria but that PVA oxidase does not.

FOOTNOTES

* Corresponding author.

Appl Environ Microbiol. 1989 February; 55(2): 275-278
Copyright © 1989, American Society for Microbiology. All Rights Reserved.





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