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Appl Environ Microbiol. 1989 February; 55(2): 488-491
Copyright © 1989, American Society for Microbiology. All Rights Reserved.

Kinetics of Acetate Utilization by Two Thermophilic Acetotrophic Methanogens: Methanosarcina sp. Strain CALS-1 and Methanothrix sp. Strain CALS-1

Hang Min{dagger} and Stephen H. Zinder*

Department of Microbiology, Stocking Hall, Cornell University, Ithaca, New York 14853-7201

ABSTRACT

The kinetics of acetate utilization were examined for washed concentrated cell suspensions of two thermophilic acetotrophic methanogens isolated from a 58°C anaerobic digestor. Progress curves for acetate utilization by cells of Methanosarcina sp. strain CALS-1 showed that the utilization rate was concentration independent (zero order) above concentrations near 3 mM and that acetate utilization ceased when a threshold concentration near 1 mM was reached. Acetate utilization by cells of Methanothrix sp. strain CALS-1 was concentration independent down to 0.1 to 0.2 mM, and threshold values of 12 to 21 µM were observed. Typical utilization rates in the concentration-independent stage were 210 and 130 nmol min–1 mg of protein–1 for the methanosarcina and the methanothrix, respectively. These results are in agreement with a general model in which high acetate concentrations favor Methanosarcina spp., while low concentrations favor Methanothrix spp. However, acetate utilization by these two strains did not follow simple Michaelis-Menton kinetics.

FOOTNOTES

* Corresponding author.

{dagger} Present address: Department of Environmental Science, Zhejiang Agricultural University, Hangzhou, People's Republic of China.

Appl Environ Microbiol. 1989 February; 55(2): 488-491
Copyright © 1989, American Society for Microbiology. All Rights Reserved.



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