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Appl Environ Microbiol. 1989 March; 55(3): 689-694
Copyright © 1989, American Society for Microbiology. All Rights Reserved.

Continuous Production of Thermostable ß-Amylase with Clostridium thermosulfurogenes: Effect of Culture Conditions and Metabolite Levels on Enzyme Synthesis and Activity

A. Nipkow, G.-J. Shen and J. G. Zeikus*

1 Michigan Biotechnology Institute, 3900 Collins Road, Lansing, Michigan 48909, and Departments of Biochemistry and Microbiology, Michigan State University, East Lansing, Michigan 488242

ABSTRACT

A ß-amylase-overproducing mutant of Clostridium thermosulfurogenes was grown in continuous culture on soluble starch to produce thermostable ß-amylase. Enzyme productivity was reasonably stable over periods of weeks to months. The pH and temperature optima for ß-amylase production were pH 6.0 and 60°C, respectively. Enzyme concentration was maximized by increasing biomass concentration by using high substrate concentrations and by maintaining a low growth rate. ß-Amylase concentration reached 90 U ml–1 at a dilution rate of 0.07 h–1 in a 3% starch medium. A further increase in enzyme activity levels was limited by acetic acid inhibition of growth and low ß-amylase productivity at low growth rates.

FOOTNOTES

* Corresponding author.

Appl Environ Microbiol. 1989 March; 55(3): 689-694
Copyright © 1989, American Society for Microbiology. All Rights Reserved.





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