This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Rodrigues, U M
Right arrow Articles by Kroll, R G
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rodrigues, U M
Right arrow Articles by Kroll, R G
Agricola
Right arrow Articles by Rodrigues, U M
Right arrow Articles by Kroll, R G

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1989 April; 55(4): 778-787

Microcolony epifluorescence microscopy for selective enumeration of injured bacteria in frozen and heat-treated foods.

U M Rodrigues and R G Kroll

Department of Microbiology, AFRC Institute of Food Research, Reading Laboratory, Shinfield, United Kingdom.

ABSTRACT

A rapid (less than 6 h) method for selectively enumerating coliforms, pseudomonads, and staphylococci has been developed which involves counting microcolonies grown on the surface of polycarbonate membranes under selective conditions. The method was not directly applicable to foods containing injured bacteria due to the poor formation of or an inability to form microcolonies under selective conditions. However, the introduction of a 3- to 5-h resuscitation step in tryptone soya broth allowed the method to give reliable estimates of these organisms in a variety of frozen and heat-processed foods. Under nonselective conditions, i.e., for total counts, the microcolony method enabled a rapid count to be made of viable bacteria in heat-treated foods, but these results were also made more consistent by the introduction of a resuscitation step. This method makes results from these foods available far faster than conventional enumeration methods.

Appl Environ Microbiol. 1989 April; 55(4): 778-787






Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»