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Appl Environ Microbiol. 1989 April; 55(4): 852-855

pH-controlled continuous cultivation of mycoplasmas.

B Krebs, M Schütz, M Fischer, G Sommer and H Kirchhoff

Institut für Mikrobiologie und Tierseuchen, Tierärztliche Hochschule Hannover, Federal Republic of Germany.

ABSTRACT

The continuous cultivation of mycoplasmas in a pH-controlled metabolistat was investigated with the fermentative strain Mycoplasma mobile 163K and the nonfermentative strain Mycoplasma arthritidis ISR1. The addition of medium and the removal of culture suspension were regulated by acid production from glucose by M. mobile 163K and by ammonium production from arginine by M. arthritidis ISR1, respectively. For both strains the optimal pH for continuous growth was 7.0. The steady state could be maintained for at least 21 days. With CFU of 8.4 X 10(9) ml-1 (M. mobile 163K) and 3.2 X 10(9) ml-1 (M. arthritidis ISR1), the cell concentrations were slightly higher than those obtained in batch cultures. The dependence on the adjusted pH values was measured for several parameters, such as flow rate, CFU, glucose fermentation or production of ammonia, and gliding velocity. Since the long lag phases of batch cultures can be avoided, pH-controlled continuous cultures provide an appropriate system for the production of mycoplasma cells.

Appl Environ Microbiol. 1989 April; 55(4): 852-855






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