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Appl Environ Microbiol. 1989 May; 55(5): 1049-1051
Copyright © 1989, American Society for Microbiology. All Rights Reserved.

Comparative Replication of Lymantria dispar Nuclear Polyhedrosis Virus Strains in Three Continuous-Culture Cell Lines

D. E. Lynn^*, E. M. Dougherty, J. T. McClintock and M. Shapiro

Insect Pathology Laboratory, BARC-West, Building 011A, Room 214, Agricultural Research Service, U.S. Department of Agriculture, Beltsville, Maryland 20705

ABSTRACT

We compared the replication of the gypsy moth (Lymantria dispar) nuclear polyhedrosis virus in two new cell lines, from embryos and fat body of L. dispar, and in a previously available ovarian cell line. Three virus isolates (the Hamden strain [LDP-67] used commercially as GYPCHEK, a plaque-purified clone of Hamden [5-7d], and an isolate from Abington, Mass. [Ab]) were each tested on the three cell lines. The fat-body-derived cell line proved best in terms of occlusion body production for all three virus strains, with the highest yield produced by the Abington strain. On the basis of these results, we conclude that a more efficient in vitro production of gypsy moth virus can be obtained by using the fat body cell line in conjunction with the Abington strain of the virus.

^* Corresponding author.

Present address: Digene Diagnostics, Inc., University of Maryland, College Park, MD 20742.