AEM
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Moyer, C. L.
Right arrow Articles by Morita, R. Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Moyer, C. L.
Right arrow Articles by Morita, R. Y.
Agricola
Right arrow Articles by Moyer, C. L.
Right arrow Articles by Morita, R. Y.

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1989 May; 55(5): 1122-1127
Copyright © 1989, American Society for Microbiology. All Rights Reserved.

Effect of Growth Rate and Starvation-Survival on the Viability and Stability of a Psychrophilic Marine Bacterium {dagger}

Craig L. Moyer and Richard Y. Morita*

1 Department of Microbiology, College of Science, and College of Oceanography,2 Oregon State University, Corvallis, Oregon 97331-3804

ABSTRACT

Cell populations of the marine bacterium ANT-300, from either batch or continuous culture with dilution rates ranging from D = 0.015 h–1 to D = 0.200 h–1, were monitored for viability, direct counts, and optical density for 98 days under starvation conditions. Three stages of starvation survival were observed for each of the cell populations. Although direct counts remained at 2 x 107 to 3 x 107 cells ml–1 throughout the starvation period, large fluctuations occurred in cell viability during stage 1 (0 to 14 days) of starvation survival. Stage 2 (14 to 70 days) involved an overall decrease in viability for each of the cell populations; the rate of viability loss was dependent upon the growth rate. Cell viability stabilized at approximately 0.3% of the direct count in stage 3 (70 to 98 days). Long-term starvation corresponded to the prolongation of stage 3 starvation survival. Cell volumes for each of the cell populations decreased with the length of the starvation period. However, the cell volume of starved cells was also dependent more on growth rate than on the length of the time starved. We hypothesize that the cell population with the slowest growth rate is most closely representative of cells found in the oligotrophic marine environment.

FOOTNOTES

* Corresponding author.

{dagger} Technical paper no. 8736, Oregon Agricultural Experiment Station.

Appl Environ Microbiol. 1989 May; 55(5): 1122-1127
Copyright © 1989, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:



Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
J. Bacteriol. Microbiol. Mol. Biol. Rev. Eukaryot. Cell All ASM Journals

Copyright © 1989 by the American Society for Microbiology. All rights reserved.