This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Adhi, T. P.
Right arrow Articles by Crawford, D. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Adhi, T. P.
Right arrow Articles by Crawford, D. L.
Agricola
Right arrow Articles by Adhi, T. P.
Right arrow Articles by Crawford, D. L.

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1989 May; 55(5): 1165-1168
Copyright © 1989, American Society for Microbiology. All Rights Reserved.

Production of Major Extracellular Enzymes during Lignocellulose Degradation by Two Streptomycetes in Agitated Submerged Culture

Tri P. Adhi, Roger A. Korus* and Don L. Crawford

1 Departments of Chemical Engineering and of Bacteriology and Biochemistry, 2 University of Idaho, Moscow, Idaho 83843

ABSTRACT

Streptomyces viridosporus T7A and S. badius 252 were grown in 1 to 2% (wt/vol) slurry cultures with mineral salts solution containing 0.6% yeast extract and 100/200 mesh ground and extracted corn lignocellulose at 37°C. Enzyme activities rapidly increased in the first 3 to 4 days and then declined and remained at a relatively constant level. Concentrations of endoglucanase and xylanase produced by S. badius were lower than those produced by S. viridosporus. However, the lignin-peroxidase peak concentration was threefold higher than with S. viridosporus and was obtained at 9 to 10 days of incubation. By polyacrylamide gel analysis, it was determined that peroxidases from both species consisted of four enzymes, with only one, the lignin peroxidase, having high activity. A culture pH of 8.5 was preferable for lignocellulose degradation by S. badius.

FOOTNOTES

* Corresponding author.

Appl Environ Microbiol. 1989 May; 55(5): 1165-1168
Copyright © 1989, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»