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Appl Environ Microbiol. 1989 May; 55(5): 1230-1233

Molecular cloning of genes from Ruminococcus flavefaciens encoding xylanase and beta(1-3,1-4)glucanase activities.

H J Flint, C A McPherson and J Bisset

Nutrition Division, Rowett Research Institute, Bucksburn, Aberdeen, United Kingdom.

ABSTRACT

Clones expressing activity against xylan or beta(1-3,1-4)glucan (lichenan) were isolated from a library of Ruminococcus flavefaciens 17 DNA made in bacteriophage lambda EMBL3. Hybridization analyses indicated the recovery of four separate genes encoding xylanases that showed no detectable associated carboxylmethylcellulase activity. One of these genes was associated with clones that also expressed beta(1-3,1-4)glucanase and beta-xylosidase activities.

Appl Environ Microbiol. 1989 May; 55(5): 1230-1233




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