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Appl Environ Microbiol. 1989 May; 55(5): 1317-1319
Copyright © 1989, American Society for Microbiology. All Rights Reserved.

Synthetic Medium for Acetobacter xylinum That Can Be Used for Isolation of Auxotrophic Mutants and Study of Cellulose Biosynthesis

E. R. Forng{dagger}, S. M. Anderson and R. E. Cannon*

Department of Biology, University of North Carolina at Greensboro, Greensboro, North Carolina 27412-5001

ABSTRACT

Acetobacter xylinum is a bacterium that can synthesize cellulose when grown in an undefined medium containing glucose. We developed a defined minimal medium for A. xylinum that contains 1% glucose, 0.1% NH4Cl, 0.115% citric acid, 0.33% Na2HPO4, 0.01% KCl, 0.025% MgSO4. 7H2O, and 7.5 mg of nicotinamide per liter which both allows cellulose synthesis and can be used to isolate a variety of auxotrophic mutants.

FOOTNOTES

* Corresponding author.

{dagger} Present address: Department of Biology, Virginia Polytechnic Institute and State University, Blacksburg, VA 23298.

Appl Environ Microbiol. 1989 May; 55(5): 1317-1319
Copyright © 1989, American Society for Microbiology. All Rights Reserved.



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Copyright © 1989 by the American Society for Microbiology. All rights reserved.