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Appl Environ Microbiol. 1989 June; 55(6): 1549-1554
Copyright © 1989, American Society for Microbiology. All Rights Reserved.

ß-Glucose-1-Phosphate, a Possible Mediator for Polysaccharide Formation in Maltose-Assimilating Lactococcus lactis

Annelie Sjöberg* and Bärbel Hahn-Hägerdal

Applied Microbiology, Chemical Center, Lund University, Post Office Box 124, S-221 00 Lund, Sweden

ABSTRACT

Homolactic fermentation of glucose and heterolactic fermentation of maltose with Lactococcus lactis 65.1 were confirmed. When moles of glucose were compared, the uptake rates of the two carbon sources were similar. The intracellular concentration of fructose-1,6-diphosphate (FDP) in maltose-assimilating cells was half of that in glucose-assimilating cells. Similarly, formation of FDP and lactate from maltose by extracts of maltose-grown cells was half of that formed from glucose by extracts of glucose-grown cells, indicating a difference in the utilization of the two carbon sources for energy metabolism. Concentrations of adenine nucleotides were similar in both types of cells. Glucose-1-phosphate was found in extracts of maltose-grown cells given maltose and, in addition, an inducible and low ß-specific phosphoglucomutase activity was observed. ß-Glucose-1-phosphate was not metabolized by cell extracts to either FDP or lactate, suggesting an alternative metabolic route. The amount of [14C]maltose incorporated into the cell material of maltose-grown cells was four times greater than that of [14C]glucose incorporated into the cell material of glucose-grown cells. The intracellular concentration of UTP was lower in maltose-assimilating cells than in glucose-assimilating cells. Cells grown on maltose were more spherical and less fragile than cells grown on glucose.

FOOTNOTES

* Corresponding author.

Appl Environ Microbiol. 1989 June; 55(6): 1549-1554
Copyright © 1989, American Society for Microbiology. All Rights Reserved.



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Copyright © 1989 by the American Society for Microbiology. All rights reserved.