This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Alaña, A. Articles by Serra, J. L. Search for Related Content PubMed PubMed Citation Articles by Alaña, A. Articles by Serra, J. L. Agricola Articles by Alaña, A. Articles by Serra, J. L.

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1989 June; 55(6): 1612-1616
Copyright © 1989, American Society for Microbiology. All Rights Reserved.

Pectin Lyase Production by a Penicillium italicum Strain

Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias, Universidad del País Vasco/Euskal Herriko Unibertsitatea, Apartado 644, E-48080 Bilbao, Spain

ABSTRACT

Growth and concomitant production of an extracellular pectin lyase (PL) [poly(methoxylgalactosiduronate) endolyase; EC 4.2.2.10] were investigated in a group of 16 fungi grown in liquid medium containing pectin as a supplementary carbon source. Culture filtrates of both Penicillium italicum (CECT 2294) and P. expansum (CECT 2275) showed the highest PL activity and contained polygalacturonase but not pectinesterase activity. The effect of the inoculum size, the carbon source (sucrose and glucose syrup), and the presence of pectin on the production of PL by P. italicum was studied. The presence of 2.6 mM glycerophosphate in the culture medium enhanced the appearance of PL but was not inhibitory for the in vitro activity. However, glycerol inhibited the enzyme nearly 50% at such a concentration.

^* Corresponding author.