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Appl Environ Microbiol. 1989 June; 55(6): 1649-1652

Transformation of Bacillus cereus vegetative cells by electroporation.

B H Belliveau and J T Trevors

Department of Environmental Biology, University of Guelph, Ontario, Canada.

ABSTRACT

Transformation of untreated vegetative cells of Bacillus cereus 569 with plasmid pC194 (1.8 megadaltons) by high-voltage electroporation resulted in a maximum of 2 x 10(-5) transformants per viable cell. Transformation of a 130-megadalton plasmid occurred at a comparable frequency. The method was simple, rapid, and yielded transformant colonies in 14 to 24 h. Transformation was obtained with unpurified total plasmid DNA.

Appl Environ Microbiol. 1989 June; 55(6): 1649-1652




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