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Appl Environ Microbiol. 1989 June; 55(6): 1653-1655

Complete nucleotide sequence and characterization of a cryptic plasmid from Lactobacillus helveticus subsp. jugurti.

R Takiguchi, H Hashiba, K Aoyama and S Ishii

Technical Research Institute, Snow Brand Products Co., Ltd., Saitama, Japan.

ABSTRACT

A small cryptic plasmid, pLJ1, was isolated from Lactobacillus helveticus subsp. jugurti and was cloned into Escherichia coli HB101 by using pBR329 as a vector. Plasmid pLJ1 was 3,292 base pairs long and had single restriction endonuclease sites for PvuII, KpnI, AvaII, Acci, HindIII, and EcoRI. In a maxicell system, pLJ1 produced a protein of about 41 kilodaltons.

Appl Environ Microbiol. 1989 June; 55(6): 1653-1655




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