This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Heitkamp, M A Articles by Cerniglia, C E Search for Related Content PubMed PubMed Citation Articles by Heitkamp, M A Articles by Cerniglia, C E Agricola Articles by Heitkamp, M A Articles by Cerniglia, C E

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1989 August; 55(8): 1968-1973

Polycyclic aromatic hydrocarbon degradation by a Mycobacterium sp. in microcosms containing sediment and water from a pristine ecosystem.

M A Heitkamp and C E Cerniglia

National Center for Toxicological Research, Food and Drug Administration, Jefferson, Arkansas 72079.

ABSTRACT

Microcosm studies were conducted to evaluate the survival and performance of a recently discovered polycyclic aromatic hydrocarbon (PAH)-degrading Mycobacterium sp. when this organism was added to sediment and water from a pristine ecosystem. Microcosms inoculated with the Mycobacterium sp. showed enhanced mineralization, singly and as components in a mixture, of 2-methylnaphthalene, phenanthrene, pyrene, and benzo[alpha]pyrene. Studies utilizing pyrene as the sole added PAH showed that the Mycobacterium sp. survived in microcosms for 6 weeks both with and without preexposure to PAH and mineralized multiple doses of pyrene. Pyrene mineralization rates for sterilized microcosms inoculated with the Mycobacterium sp. showed that competition with indigenous microorganisms did not adversely affect survival of or pyrene degradation by the Mycobacterium sp. Pyrene mineralization by the Mycobacterium sp. was not enhanced by inorganic nutrient enrichment and was hindered by organic nutrient enrichment, which appeared to result from overgrowth of indigenous bacteria. This study demonstrates the versatility of the PAH-degrading Mycobacterium sp. and expands its potential applications to include the degradation of two-, three-, four-, and five-ringed PAHs in sediments.

---

Appl Environ Microbiol. 1989 August; 55(8): 1968-1973

This article has been cited by other articles:

• Kim, S.-J., Kweon, O., Jones, R. C., Freeman, J. P., Edmondson, R. D., Cerniglia, C. E. (2007). Complete and Integrated Pyrene Degradation Pathway in Mycobacterium vanbaalenii PYR-1 Based on Systems Biology. J. Bacteriol. 189: 464-472 [Abstract] [Full Text]  
• Kim, S.-J., Kweon, O., Freeman, J. P., Jones, R. C., Adjei, M. D., Jhoo, J.-W., Edmondson, R. D., Cerniglia, C. E. (2006). Molecular Cloning and Expression of Genes Encoding a Novel Dioxygenase Involved in Low- and High-Molecular-Weight Polycyclic Aromatic Hydrocarbon Degradation in Mycobacterium vanbaalenii PYR-1. Appl. Environ. Microbiol. 72: 1045-1054 [Abstract] [Full Text]  
• Stingley, R. L., Brezna, B., Khan, A. A., Cerniglia, C. E. (2004). Novel organization of genes in a phthalate degradation operon of Mycobacterium vanbaalenii PYR-1. Microbiology 150: 3749-3761 [Abstract] [Full Text]  
• Moody, J. D., Freeman, J. P., Fu, P. P., Cerniglia, C. E. (2004). Degradation of Benzo[a]pyrene by Mycobacterium vanbaalenii PYR-1. Appl. Environ. Microbiol. 70: 340-345 [Abstract] [Full Text]  
• Moody, J. D., Fu, P. P., Freeman, J. P., Cerniglia, C. E. (2003). Regio- and Stereoselective Metabolism of 7,12-Dimethylbenz[a]anthracene by Mycobacterium vanbaalenii PYR-1. Appl. Environ. Microbiol. 69: 3924-3931 [Abstract] [Full Text]  
• Khan, A. A., Wang, R.-F., Cao, W.-W., Doerge, D. R., Wennerstrom, D., Cerniglia, C. E. (2001). Molecular Cloning, Nucleotide Sequence, and Expression of Genes Encoding a Polycyclic Aromatic Ring Dioxygenase from Mycobacterium sp. Strain PYR-1. Appl. Environ. Microbiol. 67: 3577-3585 [Abstract] [Full Text]  
• Cheung, P.-Y., Kinkle, B. K. (2001). Mycobacterium Diversity and Pyrene Mineralization in Petroleum-Contaminated Soils. Appl. Environ. Microbiol. 67: 2222-2229 [Abstract] [Full Text]  
• Moody, J. D., Freeman, J. P., Doerge, D. R., Cerniglia, C. E. (2001). Degradation of Phenanthrene and Anthracene by Cell Suspensions of Mycobacterium sp. Strain PYR-1. Appl. Environ. Microbiol. 67: 1476-1483 [Abstract] [Full Text]  
• Wang, R.-F., Wennerstrom, D., Cao, W.-W., Khan, A. A., Cerniglia, C. E. (2000). Cloning, Expression, and Characterization of the katG Gene, Encoding Catalase-Peroxidase, from the Polycyclic Aromatic Hydrocarbon-Degrading Bacterium Mycobacterium sp. Strain PYR-1. Appl. Environ. Microbiol. 66: 4300-4304 [Abstract] [Full Text]  
• Kanaly, R. A., Harayama, S. (2000). Biodegradation of High-Molecular-Weight Polycyclic Aromatic Hydrocarbons by Bacteria. J. Bacteriol. 182: 2059-2067 [Full Text]  
• Rockne, K. J., Chee-Sanford, J. C., Sanford, R. A., Hedlund, B. P., Staley, J. T., Strand, S. E. (2000). Anaerobic Naphthalene Degradation by Microbial Pure Cultures under Nitrate-Reducing Conditions. Appl. Environ. Microbiol. 66: 1595-1601 [Abstract] [Full Text]  
• Kästner, M., Streibich, S., Beyrer, M., Richnow, H. H., Fritsche, W. (1999). Formation of Bound Residues during Microbial Degradation of [14C]Anthracene in Soil. Appl. Environ. Microbiol. 65: 1834-1842 [Abstract] [Full Text]  
• Combourieu, B., Besse, P., Sancelme, M., Veschambre, H., Delort, A. M., Poupin, P., Truffaut, N. (1998). Morpholine Degradation Pathway of Mycobacterium aurum MO1: Direct Evidence of Intermediates by In Situ 1H Nuclear Magnetic Resonance. Appl. Environ. Microbiol. 64: 153-158 [Abstract] [Full Text]  
• Kästner, M., Breuer-Jammali, M., Mahro, B. (1998). Impact of Inoculation Protocols, Salinity, and pH on the Degradation of Polycyclic Aromatic Hydrocarbons (PAHs) and Survival of PAH-Degrading Bacteria Introduced into Soil. Appl. Environ. Microbiol. 64: 359-362 [Abstract] [Full Text]