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Appl Environ Microbiol. 1989 August; 55(8): 2025-2029
Copyright © 1989, American Society for Microbiology. All Rights Reserved.

Specific Dot-Immunobinding Assay for Detection and Enumeration of Thiobacillus ferrooxidans

Renato Arredondo and Carlos A. Jerez*

Departamento de Bioquímica, Facultad de Medicina, Universidad de Chile, Casilla 70086, Santiago 7, Chile

ABSTRACT

A specific and very sensitive dot-immunobinding assay for the detection and enumeration of the bioleaching microorganism Thiobacillus ferrooxidans was developed. Nitrocellulose spotted with samples was incubated with polyclonal antisera against whole T. ferrooxidans cells and then in 125I-labeled protein A or 125I-labeled goat antirabbit immunoglobulin G; incubation was followed by autoradiography. Since a minimum of 103 cells per dot could be detected, the method offers the possibility of simultaneous processing of numerous samples in a short time to monitor the levels of T. ferrooxidans in bioleaching operations.

FOOTNOTES

* Corresponding author.

Appl Environ Microbiol. 1989 August; 55(8): 2025-2029
Copyright © 1989, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Varela, P., Levicán, G., Rivera, F., Jerez, C. A. (1998). An Immunological Strategy To Monitor In Situ the Phosphate Starvation State in Thiobacillus ferrooxidans. Appl. Environ. Microbiol. 64: 4990-4993 [Abstract] [Full Text]  


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