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Appl Environ Microbiol. 1989 September; 55(9): 2113-2118

Degradation of pentachlorophenol by polyurethane-immobilized Flavobacterium cells.

K T O'Reilly and R L Crawford

Department of Bacteriology and Biochemistry, University of Idaho, Moscow 83843.

ABSTRACT

Polyurethane-immobilized Flavobacterium cells (ATCC 39723) degraded pentachlorophenol (PCP) at initial concentrations as high as 300 mg liter-1. The reversible binding of PCP to the polyurethane was shown to be important in the protection of the cells from inhibition of PCP degradation. The degradation activity of the bacteria was monitored for 150 days in semicontinuous batch reactors. The degradation rate dropped by about 0.6% per day. PCP was degraded in a continuous-culture bioreactor at a rate of 3.5 to 4 mg g of foam-1 day-1 for 25 days. Electron micrographs of the polyurethane suggested that the cells were entrapped within 50- to 500-microns-diameter pockets in the foam.

Appl Environ Microbiol. 1989 September; 55(9): 2113-2118






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