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Appl Environ Microbiol. 1990 November; 56(11): 3354-3359
Copyright © 1990, American Society for Microbiology. All Rights Reserved.

Accumulation of a Polyhydroxyalkanoate Containing Primarily 3-Hydroxydecanoate from Simple Carbohydrate Substrates by Pseudomonas sp. Strain NCIMB 40135

Geoffrey W. Haywood, Alistair J. Anderson^*, David F. Ewing and Edwin A. Dawes

¹ Departments of Applied Biology and Chemistry,² University of Hull, Hull HU6 7RX, United Kingdom

ABSTRACT

A number of Pseudomonas species have been identified which accumulate a polyhydroxyalkanoate containing mainly 3-hydroxydecanoate monomers from sodium gluconate as the sole carbon source. One of these, Pseudomonas sp. strain NCIMB 40135, was further investigated and shown to accumulate such a polyhydroxyalkanoate from a wide range of carbon sources (C₂ to C₆); however, when supplied with octanoic acid it produced a polyhydroxyalkanoate containing mainly 3-hydroxyoctanoate monomers. Polymer synthesis occurred in batch culture after cessation of growth due to exhaustion of nitrogen. In continuous culture under nitrogen limitation up to 16.9% (wt/wt) polyhydroxyalkanoate was synthesized from glucose as the carbon source. The monomer units are mainly of the R-(–) configuration. Nuclear magnetic resonance studies confirmed the composition of the polymer. Differential scanning calorimetry suggested that the solvent-extracted polymer contained a significant proportion of crystalline material. The weight-average molecular weight of the polymer from glucose-grown cells was 143,000.

^* Corresponding author.

Present address: County Analyst's Laboratory, Leicester LE7 8PF, United Kingdom.

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