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Appl Environ Microbiol. 1990 December; 56(12): 3874-3877

Cloning of the listeriolysin O gene and development of specific gene probes for Listeria monocytogenes.

Division of Microbiology, Food and Drug Administration, Washington, D.C. 20204.

ABSTRACT

A clone containing 3.1 kb of Listeria DNA was selected from a gene library of Listeria monocytogenes Scott A strain. The Escherichia coli clone produced hemolysin on sheep blood agar and in sonicated extracts but very little in the culture supernatant. This 3.1-kb DNA fragment and a 650-bp HindIII fragment located within the listeriolysin gene were used as probes in a colony hybridization assay. Both probes were specific for L. monocytogenes and did not hybridize with any other Listeria strains at high stringency. Two synthetic probes, one from the 650-bp HindIII fragment and one from the carboxy-terminal region of the protein, were also specific for L. monocytogenes.

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