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Appl Environ Microbiol. 1990 February; 56(2): 329-332

Bacterial conversion of glycerol to beta-hydroxypropionaldehyde.

J E Vancauwenberge, P J Slininger and R J Bothast

Northern Regional Research Center, U.S. Department of Agriculture, Peoria, Illinois 61604.

ABSTRACT

beta-hydroxypropionaldehyde (3-HPA) can be oxidized to acrylic acid, an industrially important chemical used in the manufacture of synthetic plastics and other polymers. Of 19 genera and 55 strains tested, 3 Klebsiella and 2 Enterobacter strains produced 3-HPA. The most efficient strain was Klebsiella pneumoniae NRRL B-4011. Under optimum conditions (28 degrees C; 40 g of semicarbazide hydrochloride per liter, 70 g of glycerol per liter; and pH 6.0), 3.1 g of B-4011 cells per liter accumulated 22 g of 3-HPA per liter at a specific rate of 0.83 g/g per h; however, 14.5 g of cells per liter accumulated 46 g of 3-HPA per liter at a specific rate of 0.41 g/g per h.


Appl Environ Microbiol. 1990 February; 56(2): 329-332







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