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Appl Environ Microbiol. 1990 February; 56(2): 340-344

Transfer of the toxin protein genes of Bacillus sphaericus into Bacillus thuringiensis subsp. israelensis and their expression.

C Bourgouin, A Delécluse, F de la Torre and J Szulmajster

Unité de Biochimie Microbienne, Institut Pasteur, Paris, France.

ABSTRACT

The genes encoding the toxic determinants of Bacillus sphaericus have been expressed in a nontoxic and a toxic strain of Bacillus thuringiensis subsp. israelensis. In both cases, the B. sphaericus toxin proteins were produced at a high level during sporulation of B. thuringiensis and accumulated as crystalline structures. B. thuringiensis transformants expressing B. sphaericus and B. thuringiensis subsp. israelensis toxins did not show a significant enhancement of toxicity against Aedes aegypti, Anopheles stephensi, and Culex pipiens larvae.


Appl Environ Microbiol. 1990 February; 56(2): 340-344




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