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Appl Environ Microbiol. 1990 June; 56(6): 1516-1517
Copyright © 1990, American Society for Microbiology. All Rights Reserved.

Simple, Sensitive Zymogram Technique for Detection of Xylanase Activity in Polyacrylamide Gels

Department of Environmental and Forest Biology, College of Environmental Science and Forestry, State University of New York, Syracuse, New York 13210

ABSTRACT

A method capable of detecting as little as 0.11 U of xylanase activity in polyacrylamide gels was developed. The method entails incubation of protein gels in contact with substrate gels containing unmodified xylan, followed by immersion of substrate gels in 95% ethanol. Resulting zymograms contain transparent bands corresponding to enzymatic activity against an opaque background.

^* Corresponding author.

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