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Appl Environ Microbiol. 1990 June; 56(6): 1763-1767
Copyright © 1990, American Society for Microbiology. All Rights Reserved.

Construction of a Simple, Inexpensive Multiple Enzyme-Linked Immunosorbent Assay Microdilution Plate Washer

Vineland Research Station, Agriculture Canada, Vineland Station, Ontario, Canada L0R 2E0

ABSTRACT

In this paper, plans are given for the construction of an inexpensive enzyme-linked immunosorbent assay plate washer from readily available materials. The wash unit uses an intermittent wash cycle based on a wash manifold cycling over the microdilution plates for a predetermined time. Laboratory tests showed that the unit provided reliable, rapid washing of plates with tap water, with no detectable contamination between wells. Substrate absorbance values for test samples from machine-washed plates were equal to or greater than absorbance values for corresponding samples from plates washed manually by an accepted protocol, by using either enzyme-linked immunosorbent assay wash buffer or tap water.