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Appl Environ Microbiol. 1990 August; 56(8): 2299-2302

Incidence of Vibrio parahaemolyticus in U.S. coastal waters and oysters.

A DePaola, L H Hopkins, J T Peeler, B Wentz and R M McPhearson

Fishery Research Branch, U.S. Food and Drug Administration, Dauphin Island, Alabama 36528.

ABSTRACT

Oyster and seawater samples were collected seasonally from May 1984 through April 1985 from shellfish-growing areas in Washington, California, Texas, Louisiana, Alabama, Florida, South Carolina, Virginia, and Rhode Island which had been designated as approved or prohibited by the National Shellfish Sanitation Program. Fecal coliforms counts, aerobic plate counts, and Vibrio parahaemolyticus densities were determined for the samples. Mean V. parahaemolyticus density was more than 100 times greater in oysters than in water, whereas density of fecal coliforms was approximately 10 times higher in oysters. Seasonal and geographical distributions of V. parahaemolyticus were related to water temperature, with highest densities in samples collected in the spring and the summer along the Gulf coast. The synthetic DNA probe for thermostable direct hemolysin hybridized with 2 of 50 isolates, 1 of which was positive by the Kanagawa test.


Appl Environ Microbiol. 1990 August; 56(8): 2299-2302




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