This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Grosser, R J
Right arrow Articles by Vestal, J R
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Grosser, R J
Right arrow Articles by Vestal, J R
Agricola
Right arrow Articles by Grosser, R J
Right arrow Articles by Vestal, J R

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1991 December; 57(12): 3462-3469

Indigenous and enhanced mineralization of pyrene, benzo[a]pyrene, and carbazole in soils.

R J Grosser, D Warshawsky and J R Vestal

Department of Biological Sciences, University of Cincinnati, Ohio 45221.

ABSTRACT

We studied the mineralization of pyrene, carbazole, and benzo[a]pyrene in soils obtained from three abandoned coal gasification plants in southern Illinois. The soils had different histories of past exposure to hydrocarbon contamination and different amounts of total organic carbon, microbial biomass, and microbial activity. Mineralization was measured by using serum bottle radiorespirometry. The levels of indigenous mineralization of 14C-labeled compounds ranged from 10 to 48% for pyrene, from undetectable to 46% for carbazole, and from undetectable to 25% for benzo[a]pyrene following long-term (greater than 180-day) incubations. Pyrene and carbazole were degraded with short or no lag periods in all soils, but benzo[a]pyrene mineralization occurred after a 28-day lag period. Mineralization was not dependent on high levels of microbial biomass and activity in the soils. Bacterial cultures that were capable of degrading pyrene and carbazole were isolated by enrichment, grown in pure culture, and reintroduced into soils. Reintroduction of a pyrene-degrading bacterium enhanced mineralization to a level of 55% within 2 days, compared with a level of 1% for the indigenous population. The carbazole degrader enhanced mineralization to a level of 45% after 7 days in a soil that showed little indigenous carbazole mineralization. The pyrene and carbazole degraders which we isolated were identified as a Mycobacterium sp. and a Xanthamonas sp., respectively. Our results indicated that mineralization of aromatic hydrocarbons can be significantly enhanced by reintroducing isolated polycyclic aromatic hydrocarbon-degrading bacteria.

Appl Environ Microbiol. 1991 December; 57(12): 3462-3469




This article has been cited by other articles:

  • Wang, X., Gai, Z., Yu, B., Feng, J., Xu, C., Yuan, Y., Lin, Z., Xu, P. (2007). Degradation of Carbazole by Microbial Cells Immobilized in Magnetic Gellan Gum Gel Beads. Appl. Environ. Microbiol. 73: 6421-6428 [Abstract] [Full Text]  
  • Uyttebroek, M., Vermeir, S., Wattiau, P., Ryngaert, A., Springael, D. (2007). Characterization of Cultures Enriched from Acidic Polycyclic Aromatic Hydrocarbon-Contaminated Soil for Growth on Pyrene at Low pH. Appl. Environ. Microbiol. 73: 3159-3164 [Abstract] [Full Text]  
  • Kim, S.-J., Kweon, O., Jones, R. C., Freeman, J. P., Edmondson, R. D., Cerniglia, C. E. (2007). Complete and Integrated Pyrene Degradation Pathway in Mycobacterium vanbaalenii PYR-1 Based on Systems Biology. J. Bacteriol. 189: 464-472 [Abstract] [Full Text]  
  • Kim, S.-J., Kweon, O., Freeman, J. P., Jones, R. C., Adjei, M. D., Jhoo, J.-W., Edmondson, R. D., Cerniglia, C. E. (2006). Molecular Cloning and Expression of Genes Encoding a Novel Dioxygenase Involved in Low- and High-Molecular-Weight Polycyclic Aromatic Hydrocarbon Degradation in Mycobacterium vanbaalenii PYR-1. Appl. Environ. Microbiol. 72: 1045-1054 [Abstract] [Full Text]  
  • Stingley, R. L., Brezna, B., Khan, A. A., Cerniglia, C. E. (2004). Novel organization of genes in a phthalate degradation operon of Mycobacterium vanbaalenii PYR-1. Microbiology 150: 3749-3761 [Abstract] [Full Text]  
  • Miyata, N., Iwahori, K., Foght, J. M., Gray, M. R. (2004). Saturable, Energy-Dependent Uptake of Phenanthrene in Aqueous Phase by Mycobacterium sp. Strain RJGII-135. Appl. Environ. Microbiol. 70: 363-369 [Abstract] [Full Text]  
  • Koivula, T. T., Salkinoja-Salonen, M., Peltola, R., Romantschuk, M. (2004). Pyrene Degradation in Forest Humus Microcosms with or without Pine and its Mycorrhizal Fungus. J. Environ. Qual. 33: 45-53 [Abstract] [Full Text]  
  • Khan, A. A., Wang, R.-F., Cao, W.-W., Doerge, D. R., Wennerstrom, D., Cerniglia, C. E. (2001). Molecular Cloning, Nucleotide Sequence, and Expression of Genes Encoding a Polycyclic Aromatic Ring Dioxygenase from Mycobacterium sp. Strain PYR-1. Appl. Environ. Microbiol. 67: 3577-3585 [Abstract] [Full Text]  
  • Cheung, P.-Y., Kinkle, B. K. (2001). Mycobacterium Diversity and Pyrene Mineralization in Petroleum-Contaminated Soils. Appl. Environ. Microbiol. 67: 2222-2229 [Abstract] [Full Text]  
  • Moody, J. D., Freeman, J. P., Doerge, D. R., Cerniglia, C. E. (2001). Degradation of Phenanthrene and Anthracene by Cell Suspensions of Mycobacterium sp. Strain PYR-1. Appl. Environ. Microbiol. 67: 1476-1483 [Abstract] [Full Text]  
  • Wang, R.-F., Wennerstrom, D., Cao, W.-W., Khan, A. A., Cerniglia, C. E. (2000). Cloning, Expression, and Characterization of the katG Gene, Encoding Catalase-Peroxidase, from the Polycyclic Aromatic Hydrocarbon-Degrading Bacterium Mycobacterium sp. Strain PYR-1. Appl. Environ. Microbiol. 66: 4300-4304 [Abstract] [Full Text]  
  • Grosser, R. J., Friedrich, M., Ward, D. M., Inskeep, W. P. (2000). Effect of Model Sorptive Phases on Phenanthrene Biodegradation: Different Enrichment Conditions Influence Bioavailability and Selection of Phenanthrene-Degrading Isolates. Appl. Environ. Microbiol. 66: 2695-2702 [Abstract] [Full Text]  
  • Bastiaens, L., Springael, D., Wattiau, P., Harms, H., deWachter, R., Verachtert, H., Diels, L. (2000). Isolation of Adherent Polycyclic Aromatic Hydrocarbon (PAH)-Degrading Bacteria Using PAH-Sorbing Carriers. Appl. Environ. Microbiol. 66: 1834-1843 [Abstract] [Full Text]  
  • Kanaly, R. A., Harayama, S. (2000). Biodegradation of High-Molecular-Weight Polycyclic Aromatic Hydrocarbons by Bacteria. J. Bacteriol. 182: 2059-2067 [Full Text]  
  • Rockne, K. J., Chee-Sanford, J. C., Sanford, R. A., Hedlund, B. P., Staley, J. T., Strand, S. E. (2000). Anaerobic Naphthalene Degradation by Microbial Pure Cultures under Nitrate-Reducing Conditions. Appl. Environ. Microbiol. 66: 1595-1601 [Abstract] [Full Text]  
  • Boonchan, S., Britz, M. L., Stanley, G. A. (2000). Degradation and Mineralization of High-Molecular-Weight Polycyclic Aromatic Hydrocarbons by Defined Fungal-Bacterial Cocultures. Appl. Environ. Microbiol. 66: 1007-1019 [Abstract] [Full Text]  
  • Kästner, M., Streibich, S., Beyrer, M., Richnow, H. H., Fritsche, W. (1999). Formation of Bound Residues during Microbial Degradation of [14C]Anthracene in Soil. Appl. Environ. Microbiol. 65: 1834-1842 [Abstract] [Full Text]  
  • Kästner, M., Breuer-Jammali, M., Mahro, B. (1998). Impact of Inoculation Protocols, Salinity, and pH on the Degradation of Polycyclic Aromatic Hydrocarbons (PAHs) and Survival of PAH-Degrading Bacteria Introduced into Soil. Appl. Environ. Microbiol. 64: 359-362 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»