This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Villarreal, D T Articles by Konopka, A Search for Related Content PubMed PubMed Citation Articles by Villarreal, D T Articles by Konopka, A Agricola Articles by Villarreal, D T Articles by Konopka, A

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1991 August; 57(8): 2135-2140

Propachlor degradation by a soil bacterial community.

Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907.

ABSTRACT

Soil from a pesticide disposal site was used to enrich for microorganisms that degraded the acylanilide herbicide propachlor (2-chloro-N-isopropylacetanilide). After seven transfers of the enrichment, the culture contained about six strains. The highest yield of microbial biomass occurred if just two of these isolates, strains DAK3 and MAB2, were inoculated into a mineral salts medium containing propachlor. When only strain DAK3 was grown on propachlor, a metabolite (2-chloro-N-isopropylacetamide) was released into the medium. Strain MAB2 could grow on this metabolite. The results of morphological and physiological tests suggest that strains DAK3 and MAB2 most closely resemble species belonging to the genera Moraxella and Xanthobacter, respectively. Strain DAK3 can respire and grow on N-substituted acylanilides containing methyl, ethyl, or isopropyl substitutions, but is incapable of respiration or growth on acetanilide, aniline, or the acylanilide herbicides alachlor and metolachlor. Strain DAK3 appears to use the aromatic C atoms of propachlor for growth, as suggested by the growth yield on propachlor and the induction of catechol 2,3-oxygenase activity in acylanilide-grown cells.

This article has been cited by other articles:

• Singh, B. K., Walker, A., Morgan, J. A. W., Wright, D. J. (2003). Role of Soil pH in the Development of Enhanced Biodegradation of Fenamiphos. Appl. Environ. Microbiol. 69: 7035-7043 [Abstract] [Full Text]  
• Martín, M., Mengs, G., Plaza, E., Garbi, C., Sánchez, M., Gibello, A., Gutierrez, F., Ferrer, E. (2000). Propachlor Removal by Pseudomonas Strain GCH1 in an Immobilized-Cell System. Appl. Environ. Microbiol. 66: 1190-1194 [Abstract] [Full Text]  
• Martin, M., Mengs, G., Allende, J. L., Fernandez, J., Alonso, R., Ferrer, E. (1999). Characterization of Two Novel Propachlor Degradation Pathways in Two Species of Soil Bacteria. Appl. Environ. Microbiol. 65: 802-806 [Abstract] [Full Text]