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Appl Environ Microbiol. 1992 January; 58(1): 1-5
Copyright © 1992, American Society for Microbiology. All Rights Reserved.

Treating Soil Solution Samplers To Prevent Microbial Removal of Analytes

David L. Lewis*, Alex P. Simons, W. Bruce Moore{dagger} and David K. Gattie

1 Environmental Research Laboratory and Technology Applications, Inc., 4 U.S. Environmental Protection Agency, Athens, Georgia 30613; Institute of Ecology, University of Georgia, Athens, Georgia 306025; Scientific Evaluation Section, Bureau of Pesticides, Department of Agriculture and Consumer Services, State of Florida, Tallahassee, Florida 323112; and Pesticide & Review Section, Department of Environmental Regulation, State of Florida, Tallahassee, Florida 323993

ABSTRACT

Soil microorganisms colonizing soil water sampling devices (lysimeters) reduced concentrations of biodegradable organic chemicals, including 2,4-dichlorophenoxyacetic acid methyl ester, alachlor, methyl m-chlorobenzoate, and metolachlor as water entered through porous ceramic cups. In some cases, losses exceeded 99%. Additions of either a biocide (sodium hypochlorite) or a bacteriostat (copper salt) prevented microbial activity so that concentrations of test chemicals inside lysimeters equaled those outside. Field studies further indicated that treating lysimeters with a copper salt effectively prevented microbial activity. Thus, chemically treating soil water samplers could improve the accuracy of soil water data for a wide variety of analytes, including environmentally important organics, such as pesticides and industrial wastes, and inorganics, such as ammonia and nitrate.


FOOTNOTES

* Corresponding author.

{dagger} {dagger} Present address: Levine-Fricke, Tallahassee, FL 32308.


Appl Environ Microbiol. 1992 January; 58(1): 1-5
Copyright © 1992, American Society for Microbiology. All Rights Reserved.







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