This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Bruce, K D Articles by Ritchie, D A Search for Related Content PubMed PubMed Citation Articles by Bruce, K D Articles by Ritchie, D A Agricola Articles by Bruce, K D Articles by Ritchie, D A

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1992 October; 58(10): 3413-3416

Amplification of DNA from native populations of soil bacteria by using the polymerase chain reaction.

Department of Genetics and Microbiology, Donnan Laboratories, University of Liverpool, United Kingdom.

ABSTRACT

Specific DNA sequences from native bacterial populations present in soil, sediment, and sand samples were amplified by using the polymerase chain reaction with primers for either "universal" eubacterial 16S rRNA genes or mercury resistance (mer) genes. With standard amplification conditions, 1.5-kb rDNA fragments from all 12 samples examined and from as little as 5 micrograms of soil were reproducibly amplified. A 1-kb mer fragment from one soil sample was also amplified. The identity of these amplified fragments was confirmed by DNA-DNA hybridization.

This article has been cited by other articles:

• Sanguin, H., Remenant, B., Dechesne, A., Thioulouse, J., Vogel, T. M., Nesme, X., Moenne-Loccoz, Y., Grundmann, G. L. (2006). Potential of a 16S rRNA-Based Taxonomic Microarray for Analyzing the Rhizosphere Effects of Maize on Agrobacterium spp. and Bacterial Communities.. Appl. Environ. Microbiol. 72: 4302-4312 [Abstract] [Full Text]  
• Ceremonie, H., Buret, F., Simonet, P., Vogel, T. M. (2004). Isolation of Lightning-Competent Soil Bacteria. Appl. Environ. Microbiol. 70: 6342-6346 [Abstract] [Full Text]  
• Newsome, T., Li, B.-J., Zou, N., Lo, S.-C. (2004). Presence of Bacterial Phage-Like DNA Sequences in Commercial Taq DNA Polymerase Reagents. J. Clin. Microbiol. 42: 2264-2267 [Abstract] [Full Text]  
• Gregory, L. G., Bond, P. L., Richardson, D. J., Spiro, S. (2003). Characterization of a nitrate-respiring bacterial community using the nitrate reductase gene (narG) as a functional marker. Microbiology 149: 229-237 [Abstract] [Full Text]  
• Miller, D. N., Bryant, J. E., Madsen, E. L., Ghiorse, W. C. (1999). Evaluation and Optimization of DNA Extraction and Purification Procedures for Soil and Sediment Samples. Appl. Environ. Microbiol. 65: 4715-4724 [Abstract] [Full Text]  
• Whitby, C. B., Saunders, J. R., Rodriguez, J., Pickup, R. W., McCarthy, A. (1999). Phylogenetic Differentiation of Two Closely Related Nitrosomonas spp. That Inhabit Different Sediment Environments in an Oligotrophic Freshwater Lake. Appl. Environ. Microbiol. 65: 4855-4862 [Abstract] [Full Text]  
• Edgcomb, V. P., McDonald, J. H., Devereux, R., Smith, D. W. (1999). Estimation of Bacterial Cell Numbers in Humic Acid-Rich Salt Marsh Sediments with Probes Directed to 16S Ribosomal DNA. Appl. Environ. Microbiol. 65: 1516-1523 [Abstract] [Full Text]  
• Hastings, R. C., Saunders, J. R., Hall, G. H., Pickup, R. W., McCarthy, A. J. (1998). Application of Molecular Biological Techniques to a Seasonal Study of Ammonia Oxidation in a Eutrophic Freshwater Lake. Appl. Environ. Microbiol. 64: 3674-3682 [Abstract] [Full Text]  
• Smit, E., Wolters, A., van Elsas, J. D. (1998). Self-Transmissible Mercury Resistance Plasmids with Gene-Mobilizing Capacity in Soil Bacterial Populations: Influence of Wheat Roots and Mercury Addition. Appl. Environ. Microbiol. 64: 1210-1219 [Abstract] [Full Text]  
• Ogunseitan, O. A. (1998). Protein Method for Investigating Mercuric Reductase Gene Expression in Aquatic Environments. Appl. Environ. Microbiol. 64: 695-702 [Abstract] [Full Text]